Relationship between three novel SNPs of BRCA1 and canine mammary tumors

The BRCA1 gene plays an important role in the development of human breast cancer, and recent research indicated that genetic variations of BRCA1 are also related to canine mammary tumors (CMTs). Here, using rapid amplification of cDNA ends (RACE), we cloned the 5′- and 3′-UTRs of BRCA1. By direct sequencing of the flanking sequences of the 5′- and 3′-UTRs of BRCA1, three previously unreported single-nucleotide polymorphisms (SNPs) were identified, two (−1228T >C, −1173C >T) in the putative promoter regions and one non-synonymous SNP (63449G >A) in exon 23. Compared with 16 normal samples, the sequences from 34 CMTs suggested that SNP (−1173C >T) was associated with the development of CMTs (odds ratio (OR)=2.57, 95% confidence interval (CI): 1.07–6.15).     

滇西北高寒地区牧草混播组合的筛选

为提高香格里拉高寒地区饲草生产能力,在小中甸镇开展了牧草混播组合试验.试验设5个混播组合(A1、A2、A3、A4、A5),随机区组设计,连续两年对牧草产量和组分进行测定,第2年取混合样对营养含量进行分析.结果表明,A1和A5组合牧草产量显著高于A2、A3和A4 (P＜0.05);A3、A1、A5年度间群落组分变化不大,稳定性较强;A2、A4、A5的粗蛋白含量较高,均超过24％.3项指标综合考虑,A1[安巴鸭茅(Dactylis glomerata cv.Anmba)+草地休衣白三叶(Trifolium repens cv.Grasslands huia)+红三叶(T.pratense)]和A5[安巴鸭茅+雅晴多年生黑麦草(Lolium perenne cv.Yatsyn)+海法白三叶(T.repens cv.Haifa)+普纳菊苣(Cichorium intybus cv.Puna)]适宜在香格里拉高寒地区种植应用.     

饲料脂肪源和乳化剂对牛蛙生长性能、肠道消化酶活力及肝脏生化指标的影响

本试验旨在研究以猪油或鱼油为脂肪源的饲料中添加乳化剂对牛蛙生长性能、肠道消化酶活力及肝脏生化指标的影响。试验采用2×2因子完全随机区组试验设计,2种脂肪源(猪油和鱼油)和2个乳化剂添加水平(0和300 mg/kg),配成4种等氮等能的试验饲料。将初始平均体重为(19.01±0.01)g的180只牛蛙随机分为4组,每组3个重复,每个重复15只蛙,进行为期8周的饲养试验。结果表明:未添加乳化剂的鱼油组增重率(WGR)和摄食量(FI)显著高于未添加乳化剂的猪油组(P0.05)。添加乳化剂的猪油组WGR和FI显著高于添加乳化剂的鱼油组(P0.05)。添加乳化剂的猪油组WGR、FI和特定生长率(SGR)显著高于未添加乳化剂的猪油组(P0.05),2组之间饲料系数(FCR)、氮沉积率(NRE)、能量沉积率(ERE)均差异不显著(P0.05)。添加乳化剂的鱼油组的各生长性能指标与未添加乳化剂的鱼油组均无显著差异(P0.05)。各组牛蛙胴体水分、粗蛋白质、粗脂肪和粗灰分含量差异不显著(P0.05),而添加乳化剂的猪油组全体粗脂肪含量显著高于未添加乳化剂的猪油组(P0.05)。各组牛蛙肠道蛋白酶和淀粉酶活力差异不显著(P0.05),饲料中添加乳化剂显著提高以猪油为主要脂肪源牛蛙的肠道脂肪酶活力(P0.05),而对以鱼油为主要脂肪源牛蛙的影响不显著(P0.05)。各组牛蛙肝脏过氧化氢酶(CAT)和超氧化物歧化酶(SOD)活力无显著差异(P0.05)。在以猪油为主要脂肪源时,添加乳化剂显著提高肝脏丙二醛(MDA)含量(P0.05);在以鱼油为主要脂肪源时,添加乳化剂对肝脏MDA含量无显著影响(P0.05)。根据结果得出,在以猪油为主要脂肪源的饲料中添加乳化剂可提高牛蛙的生长性能。     

基于斜率比法评定断奶仔猪对不同铜源的相对生物学利用率

本试验旨在研究不同铜源（硫酸铜、碱式氯化铜和柠檬酸铜）和铜添加水平（20和30 mg/kg）对断奶仔猪生长性能、血清铜含量与含铜酶活性以及组织铜含量的影响,探讨断奶仔猪对不同铜源的相对生物学利用率。试验采用3×2双因子随机区组设计,选取平均体重为（8.98±0.48） kg的杜×长×大三元杂交断奶仔猪112头,随机分为7组,每组4个重复,每个重复4头猪。对照组饲喂玉米-豆粕型基础饲粮（铜含量为7.80 mg/kg）,各试验组分别在基础饲粮中添加20或30 mg/kg硫酸铜、碱式氯化铜或柠檬酸铜（均以铜含量计）。试验预试期3 d,正试期28 d。结果表明：1）试验组与对照组断奶仔猪平均日增重、平均日采食量和料重比均无显著差异（P>0.05）。2）饲粮铜源和铜添加水平对断奶仔猪血清铜含量具有显著影响（P<0.05）,但对血清铜蓝蛋白和铜锌超氧化物歧化酶活性均无显著影响（P>0.05）。3）饲粮铜源和铜添加水平对断奶仔猪肝脏铜含量具有显著影响（P<0.05）,但对心脏、肾脏、胰脏和跖骨铜含量均无显著影响（P>0.05）。4）根据多元线性回归斜率比法计算,以血清铜...     

中日两国地区旅游形象设计比较研究及其对我国的启示

指出了地区旅游形象设计不仅仅有助于宣传当地旅游形象,而且有助于扩大本地的知名度,增进人们对本地的好感。为此,很多国家和地区都非常重视旅游形象的设计。从中国和日本的情况来看,由于传统文化思想、审美能力、设计人员的阅历和艺术创造观等诸多方面的差异,使得两国在旅游形象主题词、形象Logo和宣传口号的设计方面出现了不同的特色。针对两国已经公布的地区旅游形象设计进行了深入地剖析与阐述。通过比较研究,有助于两方取长补短,提高旅游形象设计水平,从而更好地开展宣传和扩大相关地区的知名度。     

To sea or not to sea? Multiple lines of evidence reveal the contribution of non‐diadromous recruitment for supporting endemic fish populations within New Zealand's longest river

1. Otolith microchemistry was used to identify marine‐ versus freshwater‐derived recruitment of three native freshwater fish species belonging to the southern hemisphere family Galaxiidae, in New Zealand's longest river system, the Waikato River.
2. Water chemistry data for trace elements and ⁸⁷Sr/⁸⁶Sr isotope ratios were collected from five lentic and 10 lotic water bodies throughout the lower river floodplain. Potential spawning sites for galaxiids were compared with values obtained by laser ablation inductively coupled mass spectrometry (LA‐ICPMS) depth profiling of young‐of‐the‐year otoliths sampled from fish in nine lower river catchment sites.
3. Otolith chemical signatures from the larval rearing period indicated that catchment‐scale recruitment for two species, Galaxias argenteus (Gmelin, 1789) and Galaxias fasciatus Gray, 1842, was driven predominantly by non‐diadromous recruitment from one lake (Lake Waahi). In contrast, diadromous recruitment appeared to be more common for Galaxias maculatus (Jenyns, 1842); however, non‐diadromous specimens were also identified for the first time from a New Zealand river.
4. Reversing lake outlet flows linked to river stage appears be important in facilitating the dispersal of rheotactic larvae out of lakes, suggesting that lake outflow management at key times could be used to sustain this ecologically important function.
5. This study highlights that some water bodies can supply a disproportionately large number of recruits to support fish populations within the wider riverscape. Identifying these water bodies and managing them to sustain recruitment is key to the conservation of non‐diadromous Galaxiidae in this modified lowland environment

     

Hydrological and water cycle processes of inland river basins in the arid region of Northwest China

The increasing shortage in water resources is a key factor affecting sustainable socio-economic development in the arid region of Northwest China(ARNC). Water shortages also affect the stability of the region's oasis ecosystem. This paper summarizes the hydrological processes and water cycle of inland river basins in the ARNC, focusing on the following aspects: the spatial-temporal features of water resources(including air water vapor resources, runoff, and glacial meltwater) and their driving forces; the characteristics of streamflow composition in the inland river basins; the characteristics and main controlling factors of baseflow in the inland rivers; and anticipated future changes in hydrological processes and water resources. The results indicate that:(1) although the runoff in most inland rivers in the ARNC showed a significant increasing trend, both the glaciated area and glacial ice reserves have been reduced in the mountains;(2) snow melt and glacier melt are extremely important hydrological processes in the ARNC, especially in the Kunlun and Tianshan mountains;(3) baseflow in the inland rivers of the ARNC is the result of climate change and human activities, with the main driving factors being the reduction in forest area and the over-exploitation and utilization of groundwater in the river basins; and(4) the contradictions among water resources, ecology and economy will further increase in the future. The findings of this study might also help strengthen the ecological, economic and social sustainable development in the study region.     

Abrupt temperature change and a warming hiatus from 1951 to 2014 in Inner Mongolia,China

An abrupt temperature change and a warming hiatus have strongly influenced the global climate.This study focused on these changes in Inner Mongolia, China. This study used the central clustering method, Mann-Kendall mutation test and other methods to explore the abrupt temperature change and warming hiatus in three different temperature zones of the study region based on average annual data series.Among the temperature metrics investigated, average minimum temperature(Tnav) shifted the earliest,followed by average temperature(Tnv) and average maximum temperature(Txav). The latest change was observed in summer(1990 s), whereas the earliest was observed in winter(1970 s). Before and after the abrupt temperature change, Tnav fluctuated considerably, whereas there was only a slight change in Txav.Before and after the abrupt temperature change, the winter temperature changed more dramatically than the summer temperature. Before the abrupt temperature change, Tnav in the central region(0.322°C/10 a)and west region(0.48°C/10 a) contributed the most to the increasing temperatures. After the abrupt temperature change, Tnav in winter in the central region(0.519°C/10 a) and in autumn in the west region(0.729°C/10 a) contributed the most to the temperature increases. Overall, in the years in which temperature shifts occurred early, a warming hiatus also appeared early. The three temperature metrics in spring(1991)in the east region were the first to exhibit a warming hiatus. In the east region, Txav displayed the lowest rate of increase(0.412°C/a) in the period after the abrupt temperature change and before the warming hiatus,and the highest rate of increase after the warming hiatus.